OrangerV1, France, Analysis, bibRecord, 000239

Detection and characterization of the African citrus greening Liberobacter by amplification, cloning, and sequencing of the rpl KAJL- rpo BC operon

Identifieur interne : 000239 ( France/Analysis ); précédent : 000238; suivant : 000240

Detection and characterization of the African citrus greening Liberobacter by amplification, cloning, and sequencing of the rpl KAJL- rpo BC operon

Auteurs : Pascale Planet [France] ; Sandrine Jagoueix [France] ; M. Bové [France] ; Monique Garnier [France]

Source :

Current Microbiology [ 0343-8651 ] ; 1995-03-01.

RBID : ISTEX:F361D224F83325C1CBF99750EB2A6A96050B8732

Abstract

Abstract: Greening disease of citrus is caused by a phloem-restricted, uncultured bacterium, recently characterized and named Liberobacter. As shown previously, a probe encoding ribosomal protein genes (rplKAJL-rpoBC operon) from an Asian liberobacter could detect all Asian liberobacter strains tested, but not African strains. Using the sequence of the rplKAJL-rpoBC operon of the Asian liberobacter strain from Poona (India), we have defined primers for PCR amplification of the equivalent genes of an African liberobacter strain. The amplified fragment was cloned in pUC18 and successfully used as a probe to detect African liberobacter strains by Southern and dot hybridizations. Sequence comparisons of the African and Asian liberobacter operons indicate that they represent two different species in the proposed genus Liberobacter.

Url:

https://api.istex.fr/document/F361D224F83325C1CBF99750EB2A6A96050B8732/fulltext/pdf

DOI: 10.1007/BF00296198

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 001164
to stream Istex, to step Curation: 001164
to stream Istex, to step Checkpoint: 000E96
to stream Main, to step Merge: 003856
to stream Main, to step Curation: 003589
to stream Main, to step Exploration: 003589
to stream France, to step Extraction: 000239

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ISTEX:F361D224F83325C1CBF99750EB2A6A96050B8732

Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: Greening disease of citrus is caused by a phloem-restricted, uncultured bacterium, recently characterized and named Liberobacter. As shown previously, a probe encoding ribosomal protein genes (rplKAJL-rpoBC operon) from an Asian liberobacter could detect all Asian liberobacter strains tested, but not African strains. Using the sequence of the rplKAJL-rpoBC operon of the Asian liberobacter strain from Poona (India), we have defined primers for PCR amplification of the equivalent genes of an African liberobacter strain. The amplified fragment was cloned in pUC18 and successfully used as a probe to detect African liberobacter strains by Southern and dot hybridizations. Sequence comparisons of the African and Asian liberobacter operons indicate that they represent two different species in the proposed genus Liberobacter.</div>
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   |texte=   Detection and characterization of the African citrus greening Liberobacter by amplification, cloning, and sequencing of the rpl KAJL- rpo BC operon
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Detection and characterization of the African citrus greening Liberobacter by amplification, cloning, and sequencing of the rpl KAJL- rpo BC operon

Detection and characterization of the African citrus greening Liberobacter by amplification, cloning, and sequencing of the rpl KAJL- rpo BC operon

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri